Meet

ITB Team Member

Nikolche Gjorevski

Lead Organoid Engineering

Nikolche leads the Organoid Engineering group. His research focuses on using bioengineering approaches to understand the multicellular interactions that drive tissue development and disease, and reconstitute them into better preclinical models for drug development.

List of

Publications

  1. Rezakhani, S., Gjorevski, N. & Lutolf, M. P. Extracellular matrix requirements for gastrointestinal organoid cultures. Biomaterials 276, 121020, doi:10.1016/j.biomaterials.2021.121020 (2021).

  2. Kerns, S. J. et al. Human immunocompetent Organ-on-Chip platforms allow safety profiling of tumor-targeted T-cell bispecific antibodies. Elife 10, doi:10.7554/eLife.67106 (2021).

  3. Yavitt, F. M. et al. The Effect of Thiol Structure on Allyl Sulfide Photodegradable Hydrogels and their Application as a Degradable Scaffold for Organoid Passaging. Adv Mater 32, e1905366, doi:10.1002/adma.201905366 (2020).

  4. Ragusa, S. et al. Antiangiogenic immunotherapy suppresses desmoplastic and chemoresistant intestinal tumors in mice. J Clin Invest 130, 1199-1216, doi:10.1172/JCI129558 (2020).

  5. Nikolaev, M. et al. Homeostatic mini-intestines through scaffold-guided organoid morphogenesis. Nature 585, 574-578, doi:10.1038/s41586-020-2724-8 (2020).

  6. Gjorevski, N. et al. Neutrophilic infiltration in organ-on-a-chip model of tissue inflammation. Lab Chip 20, 3365-3374, doi:10.1039/d0lc00417k (2020).

  7. Brandenberg, N. et al. High-throughput automated organoid culture via stem-cell aggregation in microcavity arrays. Nat Biomed Eng 4, 863-874, doi:10.1038/s41551-020-0565-2 (2020).

  8. Gjorevski, N. & Ordonez-Moran, P. Intestinal Stem Cell Niche Insights Gathered from Both In Vivo and Novel In Vitro Models. Stem Cells Int 2017, 8387297, doi:10.1155/2017/8387297 (2017).

  9. Gjorevski, N. & Lutolf, M. P. Synthesis and characterization of well-defined hydrogel matrices and their application to intestinal stem cell and organoid culture. Nat Protoc 12, 2263-2274, doi:10.1038/nprot.2017.095 (2017).

  10. Gjorevski, N. et al. Designer matrices for intestinal stem cell and organoid culture. Nature 539, 560-564, doi:10.1038/nature20168 (2016).

  11. Piotrowski, A. S., Varner, V. D., Gjorevski, N. & Nelson, C. M. Three-dimensional traction force microscopy of engineered epithelial tissues. Methods Mol Biol 1189, 191-206, doi:10.1007/978-1-4939-1164-6_13 (2015).

  12. Gjorevski, N., Piotrowski, A. S., Varner, V. D. & Nelson, C. M. Dynamic tensile forces drive collective cell migration through three-dimensional extracellular matrices. Sci Rep 5, 11458, doi:10.1038/srep11458 (2015).

  13. Ranga, A., Gjorevski, N. & Lutolf, M. P. Drug discovery through stem cell-based organoid models. Adv Drug Deliv Rev 69-70, 19-28, doi:10.1016/j.addr.2014.02.006 (2014).

  14. Gjorevski, N., Ranga, A. & Lutolf, M. P. Bioengineering approaches to guide stem cell-based organogenesis. Development 141, 1794-1804, doi:10.1242/dev.101048 (2014).

  15. Gjorevski, N. & Lutolf, M. Biomaterials approaches in stem cell mechanobiology. Prog Mol Biol Transl Sci 126, 257-278, doi:10.1016/B978-0-12-394624-9.00011-7 (2014).

  16. Gjorevski, N. & Nelson, C. M. Mapping of mechanical strains and stresses around quiescent engineered three-dimensional epithelial tissues. Biophys J 103, 152-162, doi:10.1016/j.bpj.2012.05.048 (2012).

  17. Gjorevski, N., Boghaert, E. & Nelson, C. M. Regulation of Epithelial-Mesenchymal Transition by Transmission of Mechanical Stress through Epithelial Tissues. Cancer Microenviron 5, 29-38, doi:10.1007/s12307-011-0076-5 (2012).

  18. Boghaert, E. et al. Host epithelial geometry regulates breast cancer cell invasiveness. Proc Natl Acad Sci U S A 109, 19632-19637, doi:10.1073/pnas.1118872109 (2012).

  19. Lee, K., Gjorevski, N., Boghaert, E., Radisky, D. C. & Nelson, C. M. Snail1, Snail2, and E47 promote mammary epithelial branching morphogenesis. EMBO J 30, 2662-2674, doi:10.1038/emboj.2011.159 (2011).

  20. Gjorevski, N. & Nelson, C. M. Integrated morphodynamic signalling of the mammary gland. Nat Rev Mol Cell Biol 12, 581-593, doi:10.1038/nrm3168 (2011).

  21. Gomez, E. W., Chen, Q. K., Gjorevski, N. & Nelson, C. M. Tissue geometry patterns epithelial-mesenchymal transition via intercellular mechanotransduction. J Cell Biochem 110, 44-51, doi:10.1002/jcb.22545 (2010).

  22. Gjorevski, N. & Nelson, C. M. Branch formation during organ development. Wiley Interdiscip Rev Syst Biol Med 2, 734-741, doi:10.1002/wsbm.96 (2010).

  23. Gjorevski, N. & Nelson, C. M. The mechanics of development: Models and methods for tissue morphogenesis. Birth Defects Res C Embryo Today 90, 193-202, doi:10.1002/bdrc.20185 (2010).

  24. Gjorevski, N. & Nelson, C. M. Endogenous patterns of mechanical stress are required for branching morphogenesis. Integr Biol (Camb) 2, 424-434, doi:10.1039/c0ib00040j (2010).

  25. Mori, H., Gjorevski, N., Inman, J. L., Bissell, M. J. & Nelson, C. M. Self-organization of engineered epithelial tubules by differential cellular motility. Proc Natl Acad Sci U S A 106, 14890-14895, doi:10.1073/pnas.0901269106 (2009).

  26. Gjorevski, N. & Nelson, C. M. Bidirectional extracellular matrix signaling during tissue morphogenesis. Cytokine Growth Factor Rev 20, 459-465, doi:10.1016/j.cytogfr.2009.10.013 (2009).